Our laboratory works at the interface
between chemistry and biology. Our research is focused on the
first step in gene expression: i.e., transcription. Our
objectives are to understand the structure, the function, and the
regulation of transcription complexes and to develop
gene-specific inhibitors of transcription as potential
gene-specific therapeutic agents.
We use a range of techniques--encompassing genetics,
biochemistry, biophysics, and combinatorial chemistry. Our core
techniques are site-specific derivatization of protein and DNA,
protein-protein and protein-DNA photocrosslinking, fluorescence
resonance polarization, and fluorescence energy transfer. Our
work to date has focused on static transcription complexes, but
we now are initiating kinetic analyses (using laser
photocrosslinking and stopped-flow fluorescence spectroscopy) and
single-molecule kinetic analyses (using optical and
scanning-probe microscopy).
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